Slot Effect Meaning
In modern physics, the double-slit experiment is a demonstration that light and matter can display characteristics of both classically defined waves and particles; moreover, it displays the fundamentally probabilistic nature of quantum mechanical phenomena. A slot is a function that is called in response to a particular signal. Qt's widgets have many pre-defined slots, but it is common practice to subclass widgets and add your own slots so that you can handle the signals that you are interested in.
Overview
Test blots, as their name implies, are very simple Western blots that are created for the express purpose of optimizing or troubleshooting experimental conditions. They are usually produced by running multiple lanes of the same lysate or purified protein solution on a gel, and after transfer cutting the blot into strips to be tested individually.
They provide a quick and efficient means of examining a range of antibody dilutions or detection substrates
Dot blots and slot blots are also a very useful variation on the typical Western blot. They do not require gel electrophoresis, so there is no separation of proteins by size.
Instead, the target protein or cell lysate mixture is added directly onto the surface of the nitrocellulose or PVDF membrane. Protein solutions can be applied directly in a small volume, or with a vacuum manifold to produce an orderly grid of samples similar to that seen in Figure 14. Each dot or slot blot would contain known amounts of target protein or cell lysate.
Once dry, dot blots and slot blots are subjected to the same immunodetection steps used for Western blotting, i.e. blocking, antibody incubation, and target detection with substrate. Grey and black spots on the figure below indicate which samples are positive for the target protein and correspond roughly to the bands produced on a Western blot.
Figure 14: Dot Blots and Slot Blots. The dot blots at the left represent a dilution series of a sample, with smaller lighter dots corresponding to lower concentrations of target protein. The slot blots represent a group of random samples, the intensity of the signal corresponds to the concentration of the target protein in that sample.
By making a number of identical dots or slots of a single known protein sample, or a range of sample dilutions, one can quickly test several combinations and concentrations of primary and secondary antibodies. Dot blots and slot blots are also beneficial when screening a large number of samples, or if a simple answer will suffice.
The main downside to slot blots/dot blots is that they provide no information about molecular weight. Thus, it is harder to detect false positive signals, or to tell whether modified forms of a protein are present.
Detection with Substrate | Chapter 4: Data Analysis |
Also found in: Thesaurus, Financial, Acronyms, Idioms, Encyclopedia, Wikipedia.
slot 1
(slŏt)n.slot 2
(slŏt)slot
(slɒt) nslot
(slɒt)slot1
(slɒt)n., v. slot•ted, slot•ting.n.
Slot Effect Meaning Synonym
slot2
(slɒt)n.
slot
Past participle: slotted
Gerund: slotting
Imperative |
---|
slot |
slot |
Present |
---|
I slot |
you slot |
he/she/it slots |
we slot |
you slot |
they slot |
Preterite |
---|
I slotted |
you slotted |
he/she/it slotted |
we slotted |
you slotted |
they slotted |
Present Continuous |
---|
I am slotting |
you are slotting |
he/she/it is slotting |
we are slotting |
you are slotting |
they are slotting |
Present Perfect |
---|
I have slotted |
you have slotted |
he/she/it has slotted |
we have slotted |
you have slotted |
they have slotted |
Past Continuous |
---|
I was slotting |
you were slotting |
he/she/it was slotting |
we were slotting |
you were slotting |
they were slotting |
Past Perfect |
---|
I had slotted |
you had slotted |
he/she/it had slotted |
we had slotted |
you had slotted |
they had slotted |
Future |
---|
I will slot |
you will slot |
he/she/it will slot |
we will slot |
you will slot |
they will slot |
Future Perfect |
---|
I will have slotted |
you will have slotted |
he/she/it will have slotted |
we will have slotted |
you will have slotted |
they will have slotted |
Future Continuous |
---|
I will be slotting |
you will be slotting |
he/she/it will be slotting |
we will be slotting |
you will be slotting |
they will be slotting |
Present Perfect Continuous |
---|
I have been slotting |
you have been slotting |
he/she/it has been slotting |
we have been slotting |
you have been slotting |
they have been slotting |
Future Perfect Continuous |
---|
I will have been slotting |
you will have been slotting |
he/she/it will have been slotting |
we will have been slotting |
you will have been slotting |
they will have been slotting |
Past Perfect Continuous |
---|
I had been slotting |
you had been slotting |
he/she/it had been slotting |
we had been slotting |
you had been slotting |
they had been slotting |
Conditional |
---|
I would slot |
you would slot |
he/she/it would slot |
we would slot |
you would slot |
they would slot |
Past Conditional |
---|
I would have slotted |
you would have slotted |
he/she/it would have slotted |
we would have slotted |
you would have slotted |
they would have slotted |
Slot Effect Meaning Synonyms
Noun | 1. | slot - a position in a grammatical linguistic construction in which a variety of alternative units are interchangeable; 'he developed a version of slot grammar' spatial relation, position - the spatial property of a place where or way in which something is situated; 'the position of the hands on the clock'; 'he specified the spatial relations of every piece of furniture on the stage' |
2. | slot - a small slit (as for inserting a coin or depositing mail); 'he put a quarter in the slot' coin slot - a slot through which coins can be inserted into a slot machine mail slot - a slot (usually in a door) through which mail can be delivered | |
3. | slot - a time assigned on a schedule or agenda; 'the TV program has a new time slot'; 'an aircraft landing slot' interval, time interval - a definite length of time marked off by two instants | |
4. | slot - a position in a hierarchy or organization; 'Bob Dylan occupied the top slot for several weeks'; 'she beat some tough competition for the number one slot' status, position - the relative position or standing of things or especially persons in a society; 'he had the status of a minor'; 'the novel attained the status of a classic'; 'atheists do not enjoy a favorable position in American life' | |
5. | slot - the trail of an animal (especially a deer); 'he followed the deer's slot over the soft turf to the edge of the trees' trail - a track or mark left by something that has passed; 'there as a trail of blood'; 'a tear left its trail on her cheek' | |
6. | slot - (computer) a socket in a microcomputer that will accept a plug-in circuit board; 'the PC had three slots for additional memory' computer, computing device, computing machine, data processor, electronic computer, information processing system - a machine for performing calculations automatically receptacle - an electrical (or electronic) fitting that is connected to a source of power and equipped to receive an insert | |
7. | slot - a slot machine that is used for gambling; 'they spend hours and hours just playing the slots' fruit machine - a coin-operated gambling machine that produces random combinations of symbols (usually pictures of different fruits) on rotating dials; certain combinations win money for the player coin machine, slot machine - a machine that is operated by the insertion of a coin in a slot | |
Verb | 1. | slot - assign a time slot; 'slot a television program' schedule - plan for an activity or event; 'I've scheduled a concert next week' |
slot
nounslot
nounA post of employment:slot
[slɒt]A.Nto put a coin in the slot → meter una monedaen laranura
to slot a part into another part → encajar una pieza en (la ranura de) otra pieza
to slot sth into place → colocar algo en su lugar
we can slot you into the programme → te podemos dar un espacioen elprograma, te podemos incluiren elprograma
it doesn't slot in with the rest → no encaja con los demás
it slots in here → entra en esta ranura, encajaaquí
slot meterN → contadorm
slot
[ˈslɒt]nto slot sth into sth → encastrer qch dans qch, insérer qch dans qch
slot
slot
[slɒt]slot
(slotSlot Effect Meaning Dictionary
) nounSlot Effect Definition
verb – past tense, past participle ˈslotted –slot
→ فَتْحَة otvor sprækkeSchlitzυποδοχήranura rakofente prorezfessuraSlot Effect Meaning Definition
スロット 동전 구멍sleufåpningszczelinafenda, ranhuraщель öppning ช่องที่แคบและยาวyuva khe狭槽Want to thank TFD for its existence? Tell a friend about us, add a link to this page, or visit the webmaster's page for free fun content.
Link to this page: